Incubation:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Cell Culture:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Blocking Assay:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Control:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Binding Assay:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Electrochemiluminescence:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Clinical Proteomics:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
Chemiluminescence Immunoassay:Article Title: Anti-IL1RAP antibody
Article Snippet: Cytokines IFN, IL-6, IL-8, TNFα were measured using the MULTI-ARRAY® technology from Meso Scale Discovery.
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 °C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Bispecific anti-CCL2 antibodies
Article Snippet: 3 ug/mL of anti-CCL2 antibody 2F2-SG1 was immobilized onto a MULTI-ARRAY 96-well plate (Meso Scale Discovery) overnight before incubating in blocking buffer for 2 hours at 30° C. Human CCL2 calibration curve samples, quality control samples and diluted mouse serum samples were incubated with denaturing buffer consisting of either 9% SDS for 30 minutes at 37° C., or pH2.0-2.5 Glycine HCl buffer for 10 minutes at 37° C. The purpose of the denaturing buffer is to dissociate human CCL2 from the biparatopic antibody.
Article Title: Human monocarboxylate transporter 1 antibodies and uses thereof
Article Snippet: A 96-well multi-array plate (Meso Scale Diagnostics, Cat. #L15XA-3) was coated at 4° C. overnight with 1 μg/mL of goat anti human FC in phosphate buffered saline (PBS).
Article Title: Influence of Biological Sex and Congenital Iron Deficiency on Neonatal Cytokine Responses.
Article Snippet: After overnight incubation, CBMC supernatants were initially frozen with liquid nitrogen, stored at −80 ◦C, and assayed later for INF-γ, IL-1β, IL-5, IL-6, IL-8, IL-10, IL-12, and TNF-α using a Human Proinflammatory multi-plex array (Meso Scale Discovery, Rockville, MD, USA).
Article Title: Clinical immunogenicity outcomes from GENEr8-1, a phase 3 study of valoctocogene roxaparvovec, an AAV5-vectored gene therapy for hemophilia A.
Article Snippet: 330 331 Bridging electrochemiluminescence assay for TAb 332 AAV5 TAb was assayed in human plasma using a validated semi-quantitative bridging 333 chemiluminescence assay with the MULTI-ARRAY Assay System (Meso Scale Diagnostics, 334 LLC, Gaithersburg, MD, USA), as previously described.27 Briefly, assay plates were coated with 335 unlabeled AAV5 capsid containing an irrelevant green fluorescent protein gene (AAV5-GFP; for 336 measuring pre-existing AAV5 TAb) or valoctocogene roxaparvovec (for measuring post-dose 337 AAV5 TAb), washed, blocked with assay diluent, and incubated with plasma samples (diluted 338 1:20).
Article Title: Anti-CTLA-4 antibodies and uses thereof
Article Snippet: Approximately 2.0×104 VI-fibroblast/hCTLA-4 cells or 1.0×104 Jurkat/NFAT chimera cells were seeded separately onto 96-well carbon electrode plates (MULTI-ARRAY high bind plate, Meso Scale Discovery (MSD; Rockville, MD)) and incubated for 1 hour (h) at 37° C. Nonspecific binding sites were blocked by 2% BSA (w/v) in PBS for 1 hour at room temperature (RT).
|
